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FAQ
What is Prime Editing?
Prime Editing is a novel gene editing technology that enables precise gene editing without introducing double-strand DNA breaks. It has two core components: pegRNA and the PEmax gene-editing enzyme (Cas9n-RT). PegRNA not only targets the desired sequence but also contains the base modification information. In the editing system, pegRNA guides PEmax to the designated edit site, nicks the DNA single strand, and reverse transcribes the sequence within the pegRNA to modify, inserting it into the target genome , thereby achieving precise single-base substitutions or small insertions and deletions
How does EDITGENE’s Bingo™ Prime Editing 7 (PE7) platform ensure point mutation success rates?
EDITGENE’s Bingo™ Prime Editing 7 (PE7) platform is built upon over ten years of gene editing experience, with optimization and advancements derived from thousands of gene editing CRO projects, achieving significantly higher success rates than traditional site-specific mutation systems. The Bingo™ Prime platform utilizes highly efficient reverse transcriptase and precise guide RNA design, ensuring each point mutation reaches the desired outcome.
How is Prime Editing 7 (PE7) different from traditional CRISPR/Cas9 technology?
Traditional CRISPR/Cas9 technology achieves gene editing by introducing double-strand breaks at the target DNA site and then using the cell’s homologous recombination repair mechanism. This approach carries multiple risks, such as lower editing efficiency, reduced homozygous mutation rates, and random insertions or deletions. Prime Editing, however, does not require double-strand breaks. With its Cas9n-RT editing enzyme system and pegRNA, Prime Editing achieves more accurate and safer gene editing with reduced off-target effects.
Are all types of genes suitable for KO cell lines?
Not all genes are suitable for knockout. Some gene knockouts may result in cell death or severe dysfunction, particularly for essential genes. In such cases, conditional knockouts or gene knockdowns (e.g., RNAi) may be used instead.
Why choose EDITGENE?
EDITGENE provides access to a comprehensive library of over 4,500 high-quality knockout (KO) cell lines, enabling researchers to save valuable time. Our custom gene knockout services are highly efficient, boasting a high positive rate while minimizing off-target effects. Clients also benefit from personalized, one-on-one support from a team of PhD experts from globally renowned institutions, ensuring top-tier service and results.
What is the difference between KO cell lines and gene knockout animal models?
KO cell lines are used for in vitro experiments, suitable for high-throughput screening and cellular studies, while gene knockout animal models are used for in vivo experiments to study gene functions within an entire organism and its interaction with the environment.
Why do researchers use KO cell lines?
Researchers use KO cell lines to investigate gene functions by observing the effects of gene deletion on cellular behavior. This helps in understanding the role of genes in various processes like cell growth, metabolism, and signal transduction. KO cell lines are vital for studying diseases like cancer, genetic disorders, and neurodegenerative diseases.
What is a KO cell line?
KO (Knockout) cell line is a cell line where a specific gene has been completely removed or rendered non-functional through gene editing technologies such as CRISPR-Cas9. These cell lines are critical for understanding gene functions and disease mechanisms.
Are KO cell lines applicable to all cell types?
KO cell lines can be applied to various cell types, including cancer cells, stem cells, and primary cells, but different cell types may have varying sensitivities to gene editing, and may vary among different cell types. In certain cell types, achieving gene knockout may require optimization of transfection conditions and selection of appropriate gene-editing tools.
What is gene overexpression?
Gene overexpression refers to using various techniques to significantly increase the expression level of a specific gene in cells or organisms. This is often achieved by introducing additional gene copies or using strong promoters to drive gene expression.
Why conduct gene overexpression?
Gene overexpression aids in studying the function of specific genes, revealing their role within the organism. It is also commonly used in drug screening, vaccine development, and protein production. For example, by overexpressing a therapeutic protein, researchers can evaluate its efficacy in disease models.
为什么选择球盟会生物基因KO细胞系？
球盟会生物基因KO细胞库有4500+株高质量KO细胞系现货可供选择，可大幅度节省您的科研时间。另外球盟会生物基因可提供高效、高阳性率的基因敲除定制服务，全球知名院校博士团队一对一服务。
KO细胞系是否适合所有类型的基因？
虽然KO细胞系技术强大，但并非所有基因都适合敲除。某些基因的敲除可能导致细胞死亡或严重的生理功能障碍，特别是那些对细胞存活至关重要的基因。在这种情况下，可以选择条件性敲除或基因敲降（如RNAi）来研究这些基因的功能。
为什么研究人员要使用KO细胞系？
研究人员使用KO细胞系来解析基因功能，因为敲除基因能够揭示该基因在细胞生长、分化、代谢、信号传导等过程中的具体作用。顺利获得比较敲除基因的细胞与正常细胞的差异，科研家可以深入分析基因在疾病中的作用，进而开发新的治疗方法。例如，KO细胞系在癌症、遗传病和神经退行性疾病的研究中具有重要应用。
基因敲入在药物开发中的作用是什么?
基因敲入在药物开发中发挥了重要作用。它用于靶点验证，顺利获得将特定基因敲入细胞系或动物模型中，确认药物靶点的有效性。它还能帮助建立疾病模型，测试药物在这些模型上的疗效和安全性。基因敲入技术还支持药物筛选，顺利获得带有敲入基因的细胞系进行高通量筛选，发现潜在药物候选分子。此外，它还能揭示药物的作用机制，优化药物结构和用法。整体而言，基因敲入技术加速了药物研发过程，并提高了治疗效果和安全性。
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